Showing posts with label inversions. Show all posts
Showing posts with label inversions. Show all posts

Friday, March 7, 2014

Sampling flies above the artic circle in Norway



# posted by Maren Wellenreuther

Hanna and I are in northern Norway to sample populations of seaweed flies (Coelopa frigida). As the name indicates, these flies live on seaweed that has been washed up on the shore and form wracks. The temperature in these wrackbeds can easily be 30 degrees above the ambient temperature and it is therefore no surprise that you can find these flies thriving year round, even in these northern latitudes. We are interested in how the flies manage to survive in different habitats (e.g. gradients in salinity, exposure, extremes of temperatures), and are planning to compare populations in southern Sweden with populations in northern Norway. Comparisons will entail genomic and transcriptomics analyses of adult and larval life stages, and experiments testing fitness in different habitats. 

We arrived on Tuesday and have been studying the maps of the coastline to find stretches that are exposed to ocean swell, which is needed for the accumulation of wrack. The expansive fjords that characterize the landscape in this part of Scandinavia make is hard to get to the outer coast, and the journey from one side to the fjord to the other typically leads over numerous tunnels and bridges that seem to disappear in the sky. Snow covered mountains are framing the view wherever we go and the temperatures that we have experienced during the days have ranged from -15 to  +7. All in all, this part of the world is full of extremes and stunning beauty.


Yesterday we found our first population. We traveled all day to an exposed stretch of coastline north of the town Bodo, which is only accessible with a short ferry journey. At the very end of the fjord we found an 80 cm deep wrack, and surely enough, seaweed flies were abundant at that site too. It had just been raining heavily with gale force winds, so all adults were hiding among the wrack.


The flies were cold and did not move at all, which made sampling easy, because we could literally pick the flies up by hand and drop them into the Eppendorf’s. At the end of the sampling the rain and wind stopped, and within minutes all the flies came out and ‘basked’ in the light.


All the best to Lund and happy greetings from the Lofoten!



Wednesday, February 19, 2014

Body size & cold resistance in flies

#Posted by Maren Wellenreuther

Next week we will be reading something that is at the heart of our groups interest. The paper will deal with insects, body size, resistance to temperature and will discuss the evolutionary implications of phenotype divergence on the genetic architecture.



I will bring German cheesecake for fika!

Here is the abstract and download link

The effect of developmental temperature on the genetic architecture underlying size and thermal clines in Drosophila melanogaster and D. simulans from the east coast of Australia.
van Heerwaarden B, Sgrò CM.

Body size and thermal tolerance clines in Drosophila melanogaster occur along the east coast of Australia. However the extent to which temperature affects the genetic architecture underlying the observed clinal divergence remains unknown. Clinal variation in these traits is associated with cosmopolitan chromosome inversions that cline in D. melanogaster. Whether this association influences the genetic architecture for these traits in D. melanogaster is unclear. Drosophila simulans shows linear clines in body size, but nonlinear clines in cold resistance. Clinally varying inversions are absent in D. simulans. Line-cross and clinal analyses were performed between tropical and temperate populations of D. melanogaster and D. simulans from the east coast of Australia to investigate whether clinal patterns and genetic effects contributing to clinal divergence in wing centroid size, thorax length, wing-to-thorax ratio, cold and heat resistance differed under different developmental temperatures (18 °C, 25 °C, and 29 °C). Developmental temperature influenced the genetic architecture in both species. Similarities between D. melanogaster and D. simulans suggest clinally varying inversion polymorphisms have little influence on the genetic architecture underlying clinal divergence in size in D. melanogaster. Differing genetic architectures across different temperatures highlight the need to consider different environments in future evolutionary and molecular studies of phenotypic divergence.

http://onlinelibrary.wiley.com/doi/10.1111/j.1558-5646.2010.01196.x/pdf